The extracellular FP produced by hESC-CMs was measured by multi-electrode array (MEA) assay using Multi Channel Systems MCS GmbH (Aspenhaustrasse, Reutlingen, Germany). Clusters of contracting hESC-CMs were plated on Matrigel® coated MEA chips containing 59 titanium electrodes and 1 internal reference electrode. Stocks of CGs were diluted to various concentrations in cell cultural medium (RPMI1640 basal medium). Data was acquired with an interval of 5 minutes at baseline and post drug applications. The FPD was analysed and corrected by the beating frequency (FPDc) with Fridericia’s formula (FPDc = FPD/interspike interval1/3)39.
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