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Preparation of colloidal chitin

KS Kumaran Subramanian
DB Deivasigamani Balaraman
MP Mani Panangal
TR Tentu Nageswara Rao
EP Elumalai Perumal
AR Amutha R
AK Alagappan Kumarappan
PR Pugazhvendan Sampath Renuga
SA Suresh Arumugam
RT Rajasekar Thirunavukkarasu
WA Wilson Aruni
SA Suliman Yousef AlOmar
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A 5 g aliquot of chitin flakes was taken, mixed with 30 ml of 35.5% hydrochloric acid at 4 ºC, and incubated overnight. To this 250 ml of 50% chilled ethanol was added slowly with constant stirring at 4 °C. Colloids of chitin were observed in ethanol. This mixture was centrifuged at 10,000 rpm and the pellet was washed with distilled water till the pH reached 7.010.

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