Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.4. Differential Scanning Fluorimetry

NM Nidhi Mittal
NS Nayanika Sengupta
SM Sameer Kumar Malladi
PR Poorvi Reddy
MB Madhuraj Bhat
RR Raju S. Rajmani
KS Koen Sedeyn
XS Xavier Saelens
SD Somnath Dutta
RV Raghavan Varadarajan
request Request a Protocol
ask Ask a question
Favorite

The thermal stability of the HA ectodomains and ectofusion immunogens were determined using nano-DSF (differential scanning fluorimetry) on a Prometheus NT.48 instrument (Nano Temper) [31]. Thermal unfolding of protein samples at a concentration of 5 μM was monitored at a rate of 1 °C/min in a range from 20 °C to 90 °C. The normalized first derivative of fluorescence ratio (350 nm/330 nm) was plotted against temperature using Prism v8.4.3 (Graph Pad Software, San Diego, CA, USA).

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A