2.3. Preparation of the Complexes of Methyl-β-Cyclodextrin (MβCD):Cholesterol

7.6 mg of cholesterol was resuspended in 153 µL of chloroform:methanol (2:1) in a glass tube. The solution was mixed and dried under a nitrogen airstream. Then 256 mg of MβCD resuspended in 2 mL of water was added drop by drop to the cholesterol solution. The resulting MβCD:cholesterol (100 mM:10 mM) complexes suspension was sonicated for 20 min in RT in a water bath and was shaken overnight at 37 °C with 500 rpm. The day after MβCD:cholesterol was filtered through a 0.22 µm filter and stored at −80 °C.