HEK-Blue TLR2 Reporter Assay

CG Chao Gu
KU Katherine Upchurch
JH Joshua Horton
MW Mathew Wiest
SZ Sandra Zurawski
MM Mark Millard
RK Robert R. Kane
HJ HyeMee Joo
LM Lisa A. Miller
SO SangKon Oh
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Human TLR2-expressing HEK 293 cells (Invivogen) were incubated with anti-hDectin-1 mAb, Pam3CSK4, or anti-hDectin-1-Pam3CSK4 conjugate at indicated concentrations for 16h. Response was detected by reading the optical density (OD) at 655 nm according to manufacturer’s instruction.

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