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For TTC staining, 2% Evans blue was injected into the heart from the femoral vein after the ligation of LAD. Heart tissues were collected immediately after the injection and rinsed with ice–cold normal saline (NS). The samples were frozen at –20° C for 30 min and subsequently transversely cut into 1 mm-thick slices. These tissues were incubated with TTC at 37° C for 15 min. After fixation with formaldehyde for 24 h, the samples were visualized and photographed.
Copyright and License information: ©2021 Zhang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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