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Post-Embedding Immunogold Electron Microscopy

FL Fengming Liu
KH Kun Han
RB Robert Blair
KK Kornelia Kenst
ZQ Zhongnan Qin
BU Berin Upcin
PW Philipp Wörsdörfer
CM Cecily C. Midkiff
JM Joseph Mudd
EB Elizaveta Belyaeva
NM Nicholas S. Milligan
TR Tyler D. Rorison
NW Nicole Wagner
JB Jochen Bodem
LD Lars Dölken
BA Bertal H. Aktas
RH Richard S. Vander Heide
XY Xiao-Ming Yin
JK Jay K. Kolls
CR Chad J. Roy
JR Jay Rappaport
SE Süleyman Ergün
XQ Xuebin Qin
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Lung tissue cubes were fixed in zinc-formalin (Z-Fix) buffer, then dehydrated and embedded with Lowicryl Monostep K4M Embedding Media (Electron Microscope Sciences (EMS), Cat# 14335) at -35°C under ultraviolet (UV) rays for 72h. Ultrathin (100nm) sections were stained with anti-SARS coronavirus polyclonal antiserum (1:100, BEI Resources, Cat# NR-10361) at 4°C overnight. After washing with PBS, secondary goat anti-guinea pig IgG 6nm (1:20, EMS, Cat# 25324) was incubated for 1h at room temperature. Images were taken under FEI Tecnai G2 F30 Twin Transmission Electron Microscope.

Copyright and License information:  ©2021 Liu, Han, Blair, Kenst, Qin, Upcin, Wörsdörfer, Midkiff, Mudd, Belyaeva, Milligan, Rorison, Wagner, Bodem, Dölken, Aktas, Vander Heide, Yin, Kolls, Roy, Rappaport, Ergün and Qin
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