Author Guidelines
  1. Protocol Format
    You can submit a manuscript in any format. A page will be automatically created to host your protocol preprint after you complete the submission process. You can update the protocol whenever you wish. Readers who follow the protocol will be automatically notified when changes are made. We encourage you to follow the standard Bio-101 format when submitting your protocol to Bio-protocol. For an example, go to
  2. Please ensure that all authors of the protocol have approved the submission of the preprint.
  3. Citation
    Readers should cite the protocol and the original research article if the protocol has been validated in it.
    Example (
    1. Wu, Y., Gao, G. F. and Gao, F. (2021). Protein expression and purification.
    2. Wu, Y., Wang, F., Shen, C., Peng, W., Li, D., Zhao, C., Li, Z., Li, S., Bi, Y., Yang, Y., Gong, Y., Xiao, H., Fan, Z., Tan, S., Wu, G., Tan, W., Lu, X., Fan, C., Wang, Q., Liu, Y., Zhang, C., Qi, J., Gao, G., Gao, F. and Liu, L. (2020). A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2. Science 368(6496). DOI: 10.1126/science.abc2241
  4. All protocol preprints are distributed under the terms of CC-BY-NC or CC-BY.
  5. By submitting your protocol to Bio-101, you agree to the Bio-protocol Terms of Service.
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