Abstract
This high throughput DNA isolation protocol is used to extract DNA of high quality from plant tissues for various genetics studies, like genotyping, and mapping etc. This protocol uses the well-established CTAB extraction procedure, and has been adapted to be used with 96-well plates.
Materials and Reagents
Equipment
Procedure
Recipes
References
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as long as the DNA is still there, it should be fine no matter how long the plants have been dried. when use this protocol for other parts/tissues, make sure the tungsten carbide beads can crack them.
Even in the dry tissues, DNA is still there. So one can ground the tissue and then isolate and purify DNA.
Frankly i don't know why sodium-acetate is added to wash DNA, but my guess would be it is in order to get pure DNA by removing metabolites
This method is basically based on the classical phenol choroform DNA isolation, so it's hard to imagine that you cannot get any DNA. my suggestion would be try something like increase you sample quantity, also, pay attention to certain steps, like when you add isoproponol, make sure it mix well with the DNA solution before spinning.