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Rapid Coomassie Protein SDS-gel Staining

QY Qingrong Yan

Published: Jun 20, 2011 DOI: 10.21769/BioProtoc.83 Views: 19066

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Abstract

This is a fast gel-staining protocol (within 10 min) compared to the conventional coomassie staining with the same detecting sensitivity.

Materials and Reagents

  1. Ethanol
  2. Acetic acid
  3. Coomassie brilliant blue R250
  4. Solution I (see Recipes)
  5. Solution II (see Recipes)
  6. Coomassie stock solution (see Recipes)

Equipment

  1. Aluminum foil
  2. Rocker

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Biochemistry > Protein > Electrophoresis

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