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Rapid Coomassie Protein SDS-gel Staining
Published: Jun 20, 2011 DOI: 10.21769/BioProtoc.83 Views: 19080
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Abstract
This is a fast gel-staining protocol (within 10 min) compared to the conventional coomassie staining with the same detecting sensitivity.
Materials and Reagents
- Ethanol
- Acetic acid
- Coomassie brilliant blue R250
- Solution I (see Recipes)
- Solution II (see Recipes)
- Coomassie stock solution (see Recipes)
Equipment
- Aluminum foil
- Rocker
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Biochemistry > Protein > Electrophoresis
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