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Labeling Protein with Thiol-reactive Probes
Published: Jun 5, 2011 DOI: 10.21769/BioProtoc.82 Views: 12535
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Abstract
This protocol is used to label protein or peptide with a maleinmide or iodoacetamide conjugated fluorescent probe through the free cysteine.
Materials and Reagents
- Thiol-reactive Maleimide probes (Life Technologies, Invitrogen™)
Note: A large collection of probes are available from Invitrogen.
- Tris-(2-carboxyethyl)phosphine (TCEP) (Life Technologies, Invitrogen™, catalog number: T2556 )
- Dithiothreitol (DTT) (Life Technologies, Molecular Probes®, catalog number: D1532 )
- DMSO
- Dissolving buffer (see Recipes)
Equipment
- Aluminum foil
- Sephadex G-25 column
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Biochemistry > Protein > Labeling
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