Abstract
This method is used to inexpensively prepare home-made competent cells of E. coli. The transformation efficiency is at the high end of chemical-efficient competent cells, and close to library-efficient competent cells.
Keywords: Competent cells, E. coli, Chemical competent cells, Rubidium chloride
Materials and Reagents
Equipment
Procedure
Recipes
References
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Sorry, I don't have it.You may ask the PI if you really want an original copy.
No, i won't help.What you need is a suitable host strain that us more tolerant.I would also try low copy number plasmid and low culture temperature, e.g. 30C or even 25C.