Abstract
Stomata pores are mainly localized in the lower epidermis of plant leaves and transpirational water loss occurs through these pores. ABA inhibits light-promoted stomatal opening and promotes stomatal closure. This protocol describes how to measure stomatal apertures following ABA treatment.
Materials and Reagents
Equipment
Procedure
Recipes
References
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I forgot mention two more questionsWhat is the recomended plant age for the experiment? and, rossette leaves or cauline leaves?thanks againVladimir
Hello!Have you solved the problem in peeling the epidermis now?I'm also confused with this technique. Furthermore I would like to observe the movement of stomata in a real time manner. Do you have any recommendation please?Thanks a lotKevin