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Arabidopsis Stomatal Opening and Closing Experiment

ZZ Zhixin Zhao

Published: May 20, 2011 DOI: 10.21769/BioProtoc.74 Views: 19105

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Abstract

Stomata pores are mainly localized in the lower epidermis of plant leaves and transpirational water loss occurs through these pores. ABA inhibits light-promoted stomatal opening and promotes stomatal closure. This protocol describes how to measure stomatal apertures following ABA treatment.

Materials and Reagents

  1. ABA
  2. KCl
  3. CaCl2
  4. Iminodiacetic acid
  5. Mes-KOH
  6. Solution for stomatal opening (see Recipes)
  7. Solution for stomatal closure (see Recipes)

Equipment

  1. Light microscope
  2. Aluminium foil
  3. Culture dish

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Plant Science > Plant physiology > Tissue analysis

Plant Science > Plant developmental biology > General

Plant Science > Plant biochemistry > Plant hormone

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Hi!I have doubt about the peel experimentsHow i could do it?Whit...
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