Abstract
Renal immune complex deposition and leukocyte infiltration are characteristic of lupus nephritis in human patients and lupus-prone mice. This protocol describes how to stain frozen sections of murine kidney to study these features using fluorescent microscopy. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.
Keywords: Lupus, Nephrtitis, Mouse, Immunofluorescent, Frozen
Materials and Reagents
Equipment
Procedure
Notes
This protocol has been successfully used for staining murine renal immune complexes, renal leukocyte infiltrates (CD4 T cells, B220 B cells, F480 macrophages, and CD11c dendritic cells), and murine splenic structures such as B cell follicles (IgD+) germinal centers (Peanut Agglutinin+).
Recipes
Acknowledgments
This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research, NY, USA. This work was supported by grants from the NY SLE Foundation (RB), Rheuminations, NIH AI082037 and AR 049938-01, NIH (PO1 AI51392 and the Flow Cytometry and Protein Expression and Tetramer Cores of PO1 AI51392).
References
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