Abstract
This method couples rabbit IgG (or any other proteins serve as affinity reagent) to the surface of magnetic beads (Brand name: Dynabeads). The amine and thiol groups of amino acid residues on the protein are covalently linked with the epoxy group on Dynabeads. The coupled IgG beads can be stored at 4°C for 6-12 month with no obvious loss in reactivity.
Keywords: Dynabeads, Immunoglobulin G, Conjugation, Immunoprecipitation, Affinity purification
Materials and Reagents
Equipment
Procedure
Day 1
Day 2
References
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