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Making Males of C. elegans   

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Abstract

Getting males from a hermaphrodite population. This is a modified version of protocol originally written by Michael Koelle at Yale University.

Materials and Reagents

  1. NGM plates

Equipment

  1. 25 and 30 °C incubators

Procedure

  1. Set up ~6 NGM plates (seeded with normal bacterial food E. coli OP50-1) with 10 L4 hermaphrodites each.
  2. Heat shock 4-6 h (no more than 6 h) at 30 °C.
  3. Move plates to 25 °C. You should get a few males per plate in the F1 generation.
    Note: To get more males, it is best to set them up with an excess of L4 hermaphrodites to ensure more males recover in the next generation.
  4. Male stocks should be maintained by selecting out an excess of males, along with a few L4 hermaphrodites in each generation (usually, male to hermaphrodite ratio is about 1: 10).
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Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: He, F. (2011). Making Males of C. elegans. Bio-101: e58. DOI: 10.21769/BioProtoc.58.
Q&A
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If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.

S M
Max Planck Institute
thank you. this is very helpful. precise and to the point.
4/22/2018 11:44:54 PM Reply
I wasn't sure about this thank you!
11/7/2012 8:25:19 AM Reply
Thanks for contributing. It's helped me undersantd the issues.
3/16/2012 10:24:22 PM Reply
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