Abstract
This protocol describes basic steps of a PCR experiment using home-made Taq DNA polymerase. Some steps may vary with different DNA polymerase.
Materials and Reagents
Equipment
Procedure
References
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Hi have a look at this article..https://www.scienceofhealthy.com/polymerase-chain-reaction-pcr/thanks
Please specify which types of gel electrophoresis (for DNA/RNA or protein?) you were asking. Since DNA/RNA gel electrophoresis is relatively simple, we do do not have such detailed protocols on our site. But, you could find many good protocols on internet, such as http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/agardna.htmlFro protein gel electrophoresis, you may search our website by using keyword "electrophoresis" and see if you could find answers that you are looking for.
I am not sure I understand your question well. To my knowledge, alkaline phosphatase is used to clean up PCR product by removing excess dNTPs (although I never used alkaline phosphatase for this purpose). So, I assume this step is done after PCR reaction.
You can request Taq polymerase plasmid from Addgene, http://www.addgene.org/25712/.
Please rephrase your question. Which step of the procedure that your question was related to?
I am not sure about what you meant by "a trailing effect from the well".