-
50 ml flask (Fisher Scientific, catalog number: 10-040C)
-
500 ml flask (PYREX, catalog number: 4980500)
-
Petri dish (Fisher Scientific, catalog number: FB0875713)
-
Wheat seeds
Triticum aestivum L. cv Chinese Spring
Triticum aestivum L. cv Amidon
Triticum aestivum L. cv Choteau
-
Agrobacterium tumefaciens strain LBA4404
-
Rifampicin (Research Products International Corp, catalog number: R640001)
-
Streptomycin (Fisher BioReagents, catalog number: BP910-50)
-
Kanamycin (Fisher BioREagents, catalog number: BP906-5)
-
pGFPGUSPlus (Addgene, Plasmid, catalog number: 64401)
-
D-Mannitol (Sigma-Aldrich, catalog number: M4125)
-
L-glutamic acid (Spectrum Chemical, catalog number: GL125)
-
Yeast extract (Caisson Labs, catalog number: Y001)
-
Tryptone (Research Products International, catalog number: 60060)
-
KH2PO4 (Sigma-Aldrich, catalog number: 1551139)
-
NaCl (Sigma-Aldrich, catalog number: S9625)
-
MgSO4·7H2O (Sigma-Aldrich, catalog number: M2773)
-
MS basal salt (Bioworld, catalog number: 30630070-1)
-
D-Maltose (Caisson labs, catalog number: M004)
-
Casein hydrolysate (Sigma-Aldrich, catalog number: 22090)
-
Myo-inositol (Caisson labs, catalog number: M014)
-
L-Proline (Caisson labs, catalog number: P015)
-
Thiamine HCl (Caisson labs, catalog number: T009)
-
Copper(II) sulfate (Sigma-Aldrich, catalog number: C1297)
-
2,4-Dichlorophenoxyacetic Acid (2,4-D) (Caisson labs, catalog number: D001)
-
X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronide) (GoldBio, catalog number: G1281)
-
Phytagel (Bioworld, catalog number: 40000035)
-
KCl (Sigma-Aldrich, catalog number: P9541)
-
Acetosyringone (Bioworld, catalog number: 40100297)
-
Timentin (Caisson labs, catalog number: T034)
-
Hygromycin (Spectrum Chemical, catalog number: H3399)
-
Indole-3-acetic Acid (IAA) (Caisson labs, catalog number: 1001)
-
6-Benzylaminopurine (6-BAP) (Caisson labs, B001)
-
Kinetin Hydrochloride (Spectrum Chemical, catalog number: K3010)
-
Lipoic Acid (spectrum Chemical, catalog number: L1515)
-
Ethanol (Sigma-Aldrich, catalog number: 16015)
-
Bleach (Clorox, catalog number: 65951360)
-
MG media (see Recipes)
-
Agrobacterium resuspension medium (see Recipes)
-
Co-cultivation media (see Recipes)
-
Callus generation media (see Recipes)
-
Resting media (see Recipes)
-
Selection media (see Recipes)
-
Regeneration media (see Recipes)
This work was supported by a grant from the Montana Wheat and Barley Committee.
The authors declare no competing interest.