Abstract
Brachypodium distachyon is a model grass closely related to wheat and barley. New resources and methods are being developed and Brachypodium is becoming a popular model for developmental biology, cell wall biology, (phylo) genomics, and genome evolution (Scholthof et al., 2018). Sterilization of seeds and seedling growth on plates is the first step to start working with Brachypodium. This is a cheap and straightforward protocol that was successfully used before (Raissig et al., 2016 and 2017).
Keywords: Brachypodium, Seed sterilization, Germination, Seedling growth on plate, Grasses
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This work was supported by the Emmy Noether Programme grant RA 3117/1-1 (to MTR). This protocol was previously established (e.g., Sauer and Burroughs, 1986) and was adapted by Emily Abrash, Michael T Raissig and Dominique C Bergmann to be used with Brachypodium seeds. Competing interests: The authors declare no conflicts of interest.
References
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