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Lentivirus Infection

NA Nabila Aboulaich

Published: Feb 20, 2011 DOI: 10.21769/BioProtoc.37 Views: 33022

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Materials and Reagents

  1. Cells
  2. Polybrene (hexadimethrine bromide) (Sigma-Aldrich, catalog number: H9268 )
  3. Puromycin
  4. Human or mouse cell line and appropriate growth media reagents required for cell-based assay
  5. Polybrene appropriated antibiotics for selection purpose

Equipment

  1. Tissue culture Incubator
  2. 6 cm tissue culture plates (Thermo Fisher Scientific)

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Molecular Biology > DNA > Transfection

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