Abstract
The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to Agrobacterium infection. The demand for developing genotype independent transformation methods is thus high. Here we report a transformation protocol for barley cultivar “Rika” by particle bombardment.
Keywords: Barley, Particle bombardment, Immature embryo, Transformation
Background
Tissue culture based Agrobacterium transformation is commonly used for introducing vector DNAs into plant cells, but it is often genotype dependent. The availability of an efficient transformation protocol is thus crucial for the success of genome editing via Agrobacterium transformation. Barley is one of the economically important cereal crops, unfortunately, Agrobacterium transformation is in principle only working for the cultivar ’Golden Promise’. Developing alternative transformation methods is thus required to facilitate genetic improvement by genome editing of the crop. Particle bombardment can deliver vector DNAs into plant cells directly, which could be an alternative transformation method for barley. Here we report a particle bombardment protocol for barley transformation modified according to wheat particle bombardment using callus (Tian et al., 2019).
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The protocols was adapted from Harwood and Smedley (2009) and Tian et al. (2019) with modifications. This work is partially financed by Einar and Inga Nilssons Foundation.
Competing interests
The author have no conflicts of interest to declare.
References
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