Abstract
RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be dramatically reduced depending on the strains and/or the conditions where cells were grown or treated. Phenol-based protocols are the most robust methods to obtain both high quantity and quality RNA from any strain of this alga grown in any condition. Here, we describe an easy and cheap protocol using phenol but avoiding the acute toxicity of guanidine isothiocyanate present in the commercial phenol-based mixtures.
Keywords: RNA, DNase treatment, Phenol extraction, Chlamydomonas
Materials and Reagents
Equipment
Procedure
Notes
Recipes
Acknowledgments
E. S-L. thanks the funding received from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie agreement No.751039. This protocol was adapted and modified from Schloss et al. (1984) and used in de Montaigu et al. (2010) and Sanz-Luque et al. (2016).
References
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