Abstract
Dinoflagellates are unicellular algae that can have photosynthetic or nonphotosynthetic lifestyles. Dinoflagellates in the genus Symbiodinium can enter endosymbiotic associations with corals, providing the metabolic basis for the highly productive and biologically diverse coral-reef ecosystems (Hoegh-Guldberg, 1999), as well as with other cnidarians, including sea anemones and jellyfish, and non-cnidarian hosts (Trench, 1993; Lobban et al., 2002; Mordret et al., 2016).Here, I describe a protocol for isolating total RNA from Symbiodinium cells.
Keywords: Symbiodinium, Coral, Symbiosis, RNA extraction, DNA
Materials and Reagents
Equipment
Procedure
Note: Things to prepare in advance.
Recipes
Acknowledgments
I thank Arthur Grossman for advice and help. This study was funded by the Gordon and Betty Moore Foundation (grant #2629). The author has no conflict of interest to declare.
References
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Hi, yes, this is perfectly suitable for zooxanthellae RNA extraction.
Thanks for your question. yes, you should use the acidic phenol solution.
Sorry, there was a typo in my previous message.Thanks for your question. yes, you can use the basic phenol solution.