Abstract
This protocol describes how to quantify different morphological defects as observed in the dopaminergic neurons using C. elegans GFP reporter strains with age.
Keywords: Dopaminergic neurons, Neurodegeneration, Aging, GFP Reporter strains, C. elegans
Materials and Reagents
Equipment
Software
Procedure
Acknowledgments
This work was supported by grants from the 2016 Buck Institute Impact Circle and the NIH (R21 AG053066) to Dr. Pankaj Kapahi. We thank Professor Michael Aschner, Professor Richard Blakely and Professor Guy Caldwell for the GFP reporter strains. Some strains were provided by the Caenorhabditis Genetics Center (funded by the NIH Office of Research Infrastructure Programs [P40 OD010440]). Conflict of Interest: The authors declare that they have no conflict of interest.
References
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