Abstract
Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model, Arabidopsis, see Lunde and Hake, 2005. For an example of how this protocol might be used for a double mutant analysis see Lunde and Hake, 2009. Here, we provide a protocol for easy DNA extraction without phenol or chloroform. Therefore, this protocol is suitable for use in schools or laboratories that lack fume hoods.
Keywords: DNA extraction, Corn, Maize, Classroom, Plant Biology
Materials and Reagents
Equipment
Procedure
Data analysis
When performing PCR, include appropriate positive (e.g., a DNA sample and primer set that has worked previously) and negative (e.g., a sample with no template) controls.
Notes
Recipes
Acknowledgments
The PCR extraction buffer recipe is a slight modification of one shared with us by Dr. Damon Lisch. CL is supported by NSF IOS-1238202, and would like to thank Dr. Sarah Hake for her continuing scientific support. SH is supported by USDA CRIS 5335-21000-013-00D. I declare that I have no conflicting interests regarding the implementation of this protocol.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.
I would be happy to give you advice. This protocol is for PCR so it's intended to be small-scale. I usually get 200-300ng/ul. Perhaps you are using a recalcitrant species? China