Abstract
Real-time quantitative PCR (qPCR) is an efficient method to detect the levels of gene expression. This protocol provides a complete and detailed procedure for qPCR, including mRNA extraction, genomic DNA removal, cDNA synthesis and targeted gene amplification steps.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was developed in Len Zon’s lab at Boston Children’s Hospital, Boston, USA, and the work was supported by NIH grant R01 HL04880-21.
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