Abstract
Double in situ hybridization I very useful to examine the relationship between the expression of two genes. But it is tricky because of the cross reaction of two different antibodies. This protocol is a valid method to do double color in situ hybridization in zebrafish embryos.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was modified from the original protocol developed in the Len Zon lab at Boston Children’s Hospital, Boston, USA and supported by NIH grant R01 HL04880-21.
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