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Subcutaneous Injection of Tumor Cells
Published: Dec 20, 2011 DOI: 10.21769/BioProtoc.166 Views: 57542
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Abstract
Growth of cells in the subcutaneous space of immunocompromised mice is a common method for assaying tumorigenic potential in vivo. This technique is also used to assess the effects of therapeutic interventions on cancer cell lines.
Materials and Reagents
- Tumor cells
- CB17 scid/scid mice
- Trypsin (Life Technologies, InvitrogenTM, catalog number: 25300-054 )
- DMEM (Life Technologies, InvitrogenTM, catalog number: 11965-092 )
- Fetal bovine serum (FBS) (Life Technologies, InvitrogenTM, catalog number: 16000-044 )
- Phosphate buffered saline (PBS)
- Trypan blue (Life Technologies, InvitrogenTM, catalog number: 15250-061 )
- Isoflurane (usually purchased through animal facility at institution)
- Matrigel (BD Biosciences, catalog number: 356234 )
- DMEM cultural medium (see Recipes)
Equipment
- Centrifuges
- Insulin syringe
- Hemocytometer
- Cell culture hood
- Incubator
- Microscope
- Small gauge
- Eppendorf tube
Procedure
Category
Cancer Biology > General technique > Animal models
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