Abstract
In this protocol, we show how to purify membrane proteins from yeast using affinity purification under native conditions at a small scale.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol has been modified and adapted in the Espenshade Lab, Johns Hopkins School of Medicine. Funding to support different projects that have used this protocol has come from NIH – National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases, the Pancreatic Cancer Action Network, and the American Heart Association.
References
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