Abstract
Glutation S-transferase (GST) tagging is the most commonly used purification strategy for recombinant protein. It was developed with the goal of preserving the enzymatic activity by utilizing gentle elution condition of the target protein from purification matrix (Poon and Hunt., 1994). The method described here can be applied from single protein to proteome scale purification of recombinant protein from yeast (Zhu et al., 2000; Zhu et al., 2001).
Materials and Reagents
Equipment
Procedure
Recipes
References
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It's a machine that holds the tube and shakes; almost like a vortexes. There are various types which can handle one or many tubes at once. Vortexer also has head accessory parts that does the similar job. However, in our hands bead beater was more effective for cell lysis.