Abstract
Protein-small molecule binding coefficients are determined by quantitative LC-MS in this method. Traditional biochemical plot is consequently used to set up the binding curve between a known protein and a small molecule (<1000 Da) to determine the affinity constant (Kd) and the stoichiometry (Bmax).
Keywords: Ligand binding, LC-MS, Molecular filtration, Protein interaction, Binding Curve
Materials and Reagents
Equipment
Procedure
References
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