实验原理:利用CTAB (十六烷基三甲基溴化铵) 作为一种阳离子去污剂,在高离子强度的溶液中与蛋白质和多聚糖形成复合物,而在低离子强度溶液中可以沉淀核酸与酸性多聚糖的特性将DNA从柑橘组织样品中分离出来。 实验目的:快速< /b>提取柑橘基因组DNA用于PCR和分子标记筛选等实验。
关键词: 柑橘, DNA, 微量提取, CTAB
材料与试剂
仪器设备
实验步骤
注意事项
溶液配方
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.