Next-generation expansion microscopy: 20× magnification in one step
Speaker: Shiwei Wang
Online live: May 20, 2025 11:00 AM EST Views: 6035
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Q&AAbstract
Expansion microscopy (ExM) has revolutionized biological imaging by enabling nanoscale-resolution visualization on conventional microscopes. Traditional ExM methods have been limited to ~4–10× expansion per step or require iterative processes to achieve higher magnifications (~15–20×). In this webinar, we will introduce 20ExM, a novel single-shot ExM protocol that achieves ~20× expansion in a single step, providing <20 nm resolution. This innovation simplifies high-resolution imaging while supporting postexpansion biomolecular staining in brain tissue. The discussion will cover the principles of ExM, the advantages of 20ExM over iterative expansion, and its potential applications in neuroscience, cell biology, and beyond.
Highlights
- Fundamentals of expansion microscopy (ExM) and its impact on high-resolution imaging.
- 20ExM: A single-shot expansion protocol achieving ~20× linear expansion.
- Post-expansion antibody staining for brain tissue and its implications.
- Applications of 20ExM in neuroscience and cell biology and comparison with previous ExM approaches.
Speaker
Shiwei Wang, B.A.
Ph.D. candidate, Massachusetts Institute of Technology
Shiwei Wang is a Ph.D. candidate in Chemical Biology at the Massachusetts Institute of Technology (MIT), where he develops innovative tools for neu...
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Keywords
Expansion microscopy (ExM), Super-resolution imaging techniques, Nanoscale visualization, Brain tissue imaging, Biomolecular labeling, Confocal microscopy
References
Wang, S., Shin, T.W., Yoder, H.B. et al. Single-shot 20-fold expansion microscopy. Nat Methods 21, 2128–2134 (2024). https://doi.org/10.1038/s41592-024-02454-9
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What advancements enable 20× magnification in a single step in next-generation expansion microscopy?
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