Improve Research Reproducibility A Bio-protocol resource

3D printing personalized tissue models

Speaker: Stephanie Willerth Moderator: Milena Restan Perez

Online live: Aug 15, 2023 12:00 PM EST Posted: Aug 22, 2023 Views: 3956

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Q&A Q&A

Abstract

3D bioprinting is a powerful tool for generating complex tissue models from stem cells. This talk will discuss recent advances in bioink technology and how they can be combined with 3D bioprinting to generate personalized tissue models.

Speaker

Stephanie Willerth

Stephanie Willerth, Ph.D.

Professor, University of Victoria

Dr. Willerth is a Full Professor in Biomedical Engineering at the University of Victoria where she has dual appointments in the Department of Mecha...

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Moderator

Milena Restan Perez

Milena Restan Perez, B.E.

Research Assistant, University of Victoria

Milena graduated with a Bachelor's in Biomedical engineering from the University of Victoria. Her research work focuses on engineering neural tissu...

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Keywords

Smart bioinks, Drug delivery, Biomaterials, Stem cells

References

1.

Restan Perez, M., Masri, N. Z., Walters-Shumka, J., Kahale, S. and Willerth, S. M. (2023). Protocol for 3D Bioprinting Mesenchymal Stem Cell–derived Neural Tissues Using a Fibrin-based Bioink. Bio-protocol 13(9): e4663. 

2.

Restan Perez M, Sharma R, Masri NZ, Willerth SM. 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. Biomolecules. 2021 Aug 21;11(8):1250. 

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46 Q&A

How does bio ink composition and printing parameters impact 3D printed tissue structure and function, and how can optimization enhance performance?

  1. What are the challenges and opportunities in scaling up 3D organ printing using bio ink technologies to facilitate the production of functional and viable organs for clinical transplantation?
  2. How does the choice of bio ink composition and printing parameters influence the structural integrity and biological functionality of 3D printed tissues and organs, and how can these factors be optimized to enhance the overall performance of the printed constructs?
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edit 0 Answer 5 Views Aug 2, 2023

Improving printability of hydrogels

What options are there to improve the printability of hydrogels, apart from mixing the hydrogel with another "more printable" hydrogel (such as alginate based hydrogels). Is there some kind of excipient that can be used to improve the viscosity of a hydrogel for example?


Kind regards,

Rammah

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edit 0 Answer 4 Views Aug 2, 2023

FFPE tissue use: Can one use tissue samples already prepared in FFPE tissue or does it have to be fresh tissue How do I preserve the tissue prior to 3

  • Can one use tissue samples already prepared in FFPE tissue or does it have to be fresh tissue
  • How do I preserve the tissue prior to 3D printing
  • Is this suitable for drug testing and how reproducible is this?


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edit 0 Answer 2 Views Aug 7, 2023

how can i corelate


What is the extent of the correlation between 3D printing personalized tissue models and their impact on liver regeneration? I'm interested in understanding the potential benefits and advancements this technology may bring to the field of liver regenerative medicine.

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edit 0 Answer 2 Views Aug 3, 2023

Grafting of pancreatic cell possible as new therapy for diabetes

Can we grow normal cells in 3 D model or will the cells turn to cellline? Can we grow pancreatic cells in this model and graft it to diabetic patients?

edit 0 Answer 2 Views Aug 3, 2023

What are the approaches to determine the compable production for an individual?

Are these approaches are a base for multi-areas of treatments? If a tissue model used as a biomarker to analyze the individual's characteristics, how to determine its future development and properties?

edit 0 Answer 2 Views Aug 3, 2023

How reliable will 3D-printed cells be for use in publications?

If 3D printing can generate cells, will it exactly mimic the gene expression and all the complex processes within the cell?

edit 0 Answer 2 Views Aug 2, 2023

Neurodegenerative disease models

can you tell us about 3d bioprinting models that can be used in neurodegenerative disease models?

edit 0 Answer 2 Views Aug 2, 2023

FFPE tissue use

  • Can one use tissue samples already prepared in FFPE tissue or does it have to be fresh tissue
  • How do I preserve the tissue prior to 3D printing
  • Is this suitable for drug testing and how reproducible is this?

Thank you

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edit 0 Answer 2 Views Aug 2, 2023

Would a viable in vitro model of the hepatic lobule be possible?

Would it be necessary to use hepatic progenitor cells? And in this case, how would it be possible to control the differentiation of these cells into hepatocytes and other cell types? 

edit 0 Answer 2 Views Aug 1, 2023

How can 3D-printed tissue models transform our study and treatment of invasive fungal infections in medical mycology?

Studying invasive fungal infections in the human body presents a significant challenge due to the complex nature of host-pathogen interactions. Current methods may not entirely capture the dynamic and multifaceted processes at play when fungal pathogens invade host tissues.

3D printing technology offers an innovative solution by creating personalized, intricate models of human tissues. These models can provide a more accurate representation of human body tissues, offering a closer look at how fungal pathogens behave and adapt within their host environment.

By utilizing these 3D models, researchers could closely mimic the infection process and analyze the host's response in a controlled setting, thereby gaining deeper insights into the mechanisms of human defense against fungal pathogens. Consequently, this could lead to more effective therapeutic strategies and improved patient outcomes.

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edit 0 Answer 1 View Aug 3, 2023