Cell-type specific sex transformations

Sex transformation constructs were made by PCR fusion of the regulatory sequences of grl-2 (ref. 34)  directly upstream of the ATG of fem-3::SL2::mCherry 18,35–37. These contructs include the unc-54 39 UTR. The grl-2 promoter included 862 bp upstream of the ATG start codon (this is a smaller region of the promoter from that used in ref. 34). We checked transgenes driven by this promoter for embryonic expression and saw no expression before the birth of the AMso. 

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