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RNA Isolation from Arabidopsis Pollen Grains

Yongxian Lu Yongxian Lu

Published: May 5, 2011 DOI: 10.21769/BioProtoc.67 Views: 18729

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Abstract

This purpose of this experiment is to isolate high quality RNAs from pollen grains, which lays the foundation for further studies, like gene expression analysis and cDNA cloning.

Materials and Reagents

  1. Mannitol (Thermo Fisher Scientific/ VWR International)
  2. TRIzol reagent (Life Technologies, InvitrogenTM)
  3. Chloroform (Thermo Fisher Scientific)
  4. 75% ethanol
  5. Liquid nitrogen
  6. RNase-free water
  7. Isopropyl alcohol

Equipment

  1. Ceramic mortar and pestles
  2. Nanodrop (Thermo Fisher Scientific)
  3. Centrifuges (Eppendorf)
  4. Vortexer (VWR International)
  5. Fume hood
  6. 500 ml flask
  7. 50 ml falcon tubes
  8. 100 μm nylon mesh

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Plant Science > Plant molecular biology > RNA > RNA extraction

Molecular Biology > RNA > RNA extraction

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1 Q&A

(1) For pollen collection, normally how many flowers are enough for RNA extraction? (2) Are the collected pollen also suitable for protein extraction?
edit 0 Answer 11 Views Mar 15, 2023