Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Peer-reviewed
Site Density Protocol
Published: Jul 5, 2012 DOI: 10.21769/BioProtoc.234 Views: 8657
PDF 
Ask a question
How to
cite
Favorite
Cited by
Abstract
The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T cell level. This method can be easily extended to quantify other surface molecule densities on different cells or beads.
Materials and Reagents
- PE-conjugated anti-mouse TCR Va2 monoclonal antibody B20.1 (BD)
- PE Rat IgG2a, λ Isotype Control (BD)
- QuantiBRITE PE beads tube (BD)
Equipment
- Countertop Centrifuge
- BD LSR flow
Procedure
Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.
Category
Immunology > Immune cell function > Lymphocyte
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
