Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Peer-reviewed
Injecting Zebrafish Embryos
Published: Jan 20, 2011 DOI: 10.21769/BioProtoc.22 Views: 19493
PDF 
Ask a question
How to
cite
Favorite
Cited by
Abstract
Zebrafish is convenient for transient genetic manipulations such as morpholino-mediated gene knockdown, DNA/mRNA-based genetic overexpression. These genetic approaches do not involve the challenge of making transgenic animals, and their effect can last up to a week. This protocol describes the detailed steps of injecting to zebrafish embryos.
Materials and Reagents
- Zebrafish
- Oil
- Zebrafish mating boxes (Aquatics)
- Mineral oil (Sigma-Aldrich, catalog number: M5904 )
Equipment
- Bench
- Forceps
- Injection
- Micrometer slide
- Micropipette puller (Sutter instrument)
- Microinjection system Pico-injector (Harvard Apparatus)
- Benchtop microscope (Leica Microsystems)
- Micrometer slide (3B Scientific)
- Glass capillary tubing (Thermo Fisher Scientific)
- Benchtop microcentrifuge (Biotool)
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > Transformation
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question1 Q&A
