Hybond-N, nylon membrane
Streptavidin-HRP (R&D systems Cat :890803)
PBS x10
20% SDS
0.5M EDTA
ECL kit
X-ray Film
Stratalinker UV cross linker
benchtop shaker
film developer
Blocking buffer: PBS x1 pH 7.4, 10% SDS, 1mM EDTA
Wash buffer 1: PBS x1 pH 7.4, 10% SDS
Wash buffer 2: PBS x1 pH 7.4, 1% SDS
Wash buffer 3: PBS x1 pH 7.4, 0.1% SDS