luminometer (veritas) in Adi Kimchi's lab
cutlure cells and treat approprietly.
Wash cells once in PBSx1
Add 100µl lysis buffer. wait 2 minutes RT then use pipetor up&down to lyse all cells in well.
Transfer 40µl x2 to black 96-well.
prepare 50ml tube with ddwater, 50ml tube with 70% ethanol, empty 50ml tube (waste) and 15ml with luciferase reaction buffer wrapped in aluminium foil [prepare 100µl/well in 96 plate + 1ml extra].
reading results:
Start the machine, then start Veritas software.
go to "saved procedure --> luciferase --> galh"
click "options" --> choice injector 1" and choose wells. press "Apply choice"
Click "Prime" --> injector 1. follow instructions ans startthe priming. at the end, return the tube to its holder.
press "Start" - the reading will now take place.
after its done, press "Flush" and follow instructions (was once in water --> ethanol --> water). make sure "flush cycle" is set to '3'.