#2 Drop-out plate medium (1 Liter)
1.7 g Yeast nitrogen base without amino-acids without ammonium sulfate
5 g Ammonium sulfate
2 g drop-out powder mix
20g Bacto Agar
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Glucose
100 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
After autoclaving mix the two solutions
Stir slowly to avoid bubbles
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
#3 Drop-out plate medium (1 Liter)
1.7 g Yeast nitrogen base without amino-acids without ammonium sulfate
5 g Ammonium sulfate
2 g drop-out powder mix
20g Bacto Agar
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Raffinose
100 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
After autoclaving mix the two solutions
Stir slowly to avoid bubbles
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
#4 Drop-out plate medium (1 Liter)
1.7 g Yeast nitrogen base without amino-acids without ammonium sulfate
5 g Ammonium sulfate
2 g drop-out powder mix
20g Bacto Agar
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Raffinose
20 g Galactose
100 mls MilliQ water
Stir, but ingridients will not dissovle
Autoclave at 121 C for 25 minutes.
After autoclaving mix the two solutions
Stir slowly to avoid bubbles
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
YPD plate medium (1 Liter)
10 g yeast extract
20 g peptone
20g Bacto Agar
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Glucose
100 mls MilliQ water
Stir, but ingridients will not dissovle
Autoclave at 121 C for 25 minutes.
For plates with antibiotics add the following:
4 ml G418 (50 mg/ml) = Kan or Geniticin
300 ul NAT (200 mg/ml) = clonNAT
4 ml Hygromycin B (50 mg/ml)
1 ml Chloramphenicol (40 mg/ml)
Let cool down on the side for 45 minutes
Add antibiotic and stir slowly to avoid bubbles
Pour plates right away 1L= 2 sleeves
Kac – sporulation plate media (1 Liter)
20 g Potassium acetate
20 g Bacto Agar
100 mls MilliQ water
Adjust to pH 7.0
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
5 FoA plate media (1 Liter)
3.35 g Yeast nitrogen base without amino-acids without ammonium sulfate
10 g glucose
1g drop-out powder mix (Complete)
1000 mls MilliQ water
Adjust to pH 7.0
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
#2 Drop-out liquid medium (1 Liter)
1.7 g Yeast nitrogen base without amino-acids without ammonium sulfate
5 g Ammonium sulfate
2 g drop-out powder mix
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Glucose
100 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
After autoclaving mix the two solutions
Stir slowly to avoid bubbles
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
#3 Drop-out liquid medium (1 Liter)
1.7 g Yeast nitrogen base without amino-acids without ammonium sulfate
5 g Ammonium sulfate
2 g drop-out powder mix
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Raffinose
100 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
After autoclaving mix the two solutions
Stir slowly to avoid bubbles
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
#4 Drop-out liquid medium (1 Liter)
1.7 g Yeast nitrogen base without amino-acids without ammonium sulfate
5 g Ammonium sulfate
2 g drop-out powder mix
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Raffinose
20 g Galactose
100 mls MilliQ water
Stir, but ingridients will not dissovle
Autoclave at 121 C for 25 minutes.
After autoclaving mix the two solutions
Stir slowly to avoid bubbles
Let cool down on the side for 45 minutes, then pour 1L= 2 sleeves
YPD liquid medium (1 Liter)
10 g yeast extract
20 g peptone
20g Bacto Agar
900 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.
20 g Glucose
100 mls MilliQ water
Stir, but ingridients will not dissovle
Autoclave at 121 C for 25 minutes.
LB plate medium (1 Liter)
10 g Tryptone
5 g Yeast Extract
10 g Sodium Chloride
15 g Bacto Agar
1000 mls MilliQ water
Stir until all ingredients are dissolved
Autoclave at 121 C for 25 minutes.