Cancel
Please leave your comments or questions
 

Fly Recombinant Protocol - Making Recombinant Using Same Insertion Chromosome Lines (3rd Chromosome Example)

Author: Jennifer Beck, updated date: , view: 444, Q&A: 0
Tags: Fly, Drosophila and Recombinant

Procedure

  1. w; +/+; P[transgeneA]/P[transgeneA] ☿☿ x w; +/+; P[transgeneB]/P[transgeneB] ♂♂
    ↓

  2. w; +/+; P[transgeneA]/P[transgeneB] ☿☿ x w; +/+; TM3, Sb/TM6B, Tb ♂♂ You will get...

    • w; P[transgeneA/TM3, Sb or TM6B, Tb (red eye)

    • w; P[transgeneB/TM2, Sb or TM6B, Tb (red eye)

    • w; +/TM3, Sb or TM6b, Tb (white eye)

    • w; P[transgeneA],P[transgeneB]/TM3, Sb or TM6B, Tb (strong or dark red eye) <-- want this fly for next cross
      ↓

  3. w; +/+; P[transgeneA],P[transgeneB]/TM3, Sb ☿ (single female) x w; +/+; TM3, Sb/TM6B, Tb ♂♂ (setup 5-10 vials with different single females)
    OR
    w; +/+; TM3, Sb/TM6B, Tb ☿ ☿ x w; +/+; P[transgeneA],P[transgeneB]/TM3, Sb ♂ (setup 5-10 vials with different single males)
    ↓

  4. w; +/+; P[transgeneA],P[transgeneB]/TM3, Sb ☿☿ x w; +/+; P[transgeneA],P[transgeneB]/TM3, Sb ♂♂
    ↓

  5. w; +/+; P[transgeneA],P[transgeneB]/P[transgeneA],P[transgeneB] ☿☿ x w; +/+; P[transgeneA],P[transgeneB]/P[transgeneA],P[transgeneB] ♂♂

Comment
Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.
You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.