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Fly Recombinant Protocol - Making Recombinant Using DIFFERENT Insertion Chromosome Lines
Author: Jennifer Beck, updated date: , view: 35, Q&A: 0
Tags: Fly, Drosophila and Recombinant

Procedure

  1. w; P[transgeneA]/P[transgeneA]; +/+ ☿☿ x w; CyO/Sco; MRKS, Sb/TM6B, Tb ♂♂
    AND
    w; +/+; P[transgeneB]/P[transgeneB] ☿☿ x w; CyO/Sco; MRKS, Sb/TM6B, Tb ♂♂
    ↓

  2. w; P[transgeneA]/Sco; +/TM6B, Tb ☿☿ x w; +/CyO; P[transgeneB]/MKRS, Sb ♂♂
    ↓

  3. w; P[transgeneA]/CyO; P[transgeneB]/TM6B, Tb ☿☿ x w; P[transgeneA]/CyO; P[transgeneB/TM6B, Tb ♂♂
    ↓

  4. w; P[transgeneA]/P[transgeneA]; P[transgeneB]/P[transgeneB] ☿☿ x w; P[transgeneA]/P[transgeneA]; P[transgeneB]/P[transgeneB] ♂♂

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