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Flow Cytometry (with Methanol)

Author: Alex Kuo, updated date: , view: 130, Q&A: 0
Tags: CyTOF and Flow Cytometry

Procedure

FLOW CYTOMETRY with TRITON

Wash cells with PBS

Trypsinize cells, collect in media, and spin down at 300g for 5 min

Resuspend in PBS and spin

Add 1 ul Zombie for 1 million cells

Incubate 15 mins in dark

Wash w/CyTOF buffer and spin

Add 4 mL 1.6% PFA in PBS and incubate for 15 mins

Add 1 mL 0.1% Triton in PBS and incubate for 20 mins

Wash twice at 600g for 5 mins

Add blocking and antibodies

Incubate overnight

Wash twice with CyTOF buffer

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