Flow Cytometry (with Methanol)

Author: Alex Kuo, updated date: , view: 266, Q&A: 0
Tags: CyTOF and Flow Cytometry

Procedure

FLOW CYTOMETRY with TRITON

Wash cells with PBS

Trypsinize cells, collect in media, and spin down at 300g for 5 min

Resuspend in PBS and spin

Add 1 ul Zombie for 1 million cells

Incubate 15 mins in dark

Wash w/CyTOF buffer and spin

Add 4 mL 1.6% PFA in PBS and incubate for 15 mins

Add 1 mL 0.1% Triton in PBS and incubate for 20 mins

Wash twice at 600g for 5 mins

Add blocking and antibodies

Incubate overnight

Wash twice with CyTOF buffer

Comment

Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.

You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.