Materials and Reagents
PCR supplies
Pfu Turbo (Stratagene, now Agilent)
Equipment
PCR machine
PCR tubes
PCR tube rack
Procedure
PCR
Add the components into a PCR tube
Don't forget to perform a control reaction without primers
Run the following PCR protocol using a PCR machine
After PCR has run transfer 30uL to a fresh microfuge tube
Add 1uL of DpnI
Incubate at 37C for 1 hr.
During this incubation take 1uL of the remaining 20uL of PCR to run on an agarose gel.
Take 2uL of DpnI-digested mixture and transform into bacteria (don't forget to transform the control reaction that did not have primers).
References
This protocol is adapted from the PCR PROTOCOL FOR PfuTURBO DNA POLYMERASE by Stratagene (early 2000's).