Please leave your comments or questions

Pierce BCA Protein Assay Kit (23225/25227)

Author: Jennifer Beck, updated date: , view: 1186, Q&A: 0
Tags: Fly, Protein, Drosophila and Spectraphotometer


  1. BSA = 2 mg/ml

  2. Standard Curve: (serial dilution) 

    stdBSA (2 mg/ml)H2OValues
    510 ul0 ul2 ug/ul
    45 ul5 ul1 ug/ul
    32.5 ul7.5 ul0.5 ug/ul
    21.25 ul8.75 ul0.25 ug/ul
    10.6259.375 ul0.125 ug/ul
    Blank010 ul0 ug/ul
  3. Sample dilution:

    • 5 ul of protein in each (for duplicate reading) 

  4. Reagent dilution: (per plate)

    • 50:1 :: BCA:CuSO4. Prepare appropriate volume for 90ul per sample. 

      • BCA 

        • 1 plate = 96 wells

        • 96 wells x 95 ul = 9120ul = 9.2ml

      • CuSO4

        • (9.2 ml BCA)(1 ul CuSO4/50ul BCA) 

        • (9.2 ml)/(50 ul) = 184 ul CuSO4

  5. Incubate in dark for 30 min at 37°C. 

  6. Read in multi-reader at 570 nm.

Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.
You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.