Procedure
Stock Solution:
10 mM Tris-HCl (pH 8.2), 1 mM EDTA, 25 mM NaCl
100 ug/ml Proteinase K (add just before use)
DNA extraction buffer:
Proteinase K (10 mg/ml)
Method:
Put fly into 1.5 ml tube and keep on ice.
Crush with 50 ul of DNA extraction buffer using pipet tip
Centrifuge for 1 min at maximum speed
Transfer supernatant in PCR tube
Incubate for 30 min at 37°C
Incubate for 5 min at 95°C
Puton ice immediately
Store at -20°C
PCR Protocol:
Cycler: