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Mowiwol Preparation

Author: Amit Sharma, updated date: , view: 1097, Q&A: 0
Tags: Cell Culture and Microscopy

Materials and Reagents

  1. Mowiol (Mowiol 4.88, Calbiochem catalog number 475904)

  2. glycerol

  3. NaN3 (Sodium Azide)

Procedure

  1. Add 6 g glycerol in a 50 ml plastic centrifuge tube and mix will using a small stir bar on megnatic sterring.

  2. Add 2.4 g Mowiol (Calbiochem); stir to mix.

  3. While stirring, add 6 ml distilled water and leave 2 hours at room temperature

  4. Add 12 ml 0.2M Tris (pH 8.5).

  5. Add a small amount of NaN3 (Sodium Azide) such that the final concentration is .02% (optional).

  6. Incubate the tube in hot water (50 – 60ºC) for 10 minutes to dissolve the Mowiol. This can be repeated over several hours if necessary

  7. Centrifuge at 5000 g for 15 minutes to remove any undissolved solids. Store 1ml aliquots in eppendorf tubes at -20ºC.

  8. Warm tubes to room temperature for use. Opened tubes can be stored at 4ºC for approximately 1 month. Discard if any crystalline material is seen in the tube or on the slides.


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