×
Please leave your comments or questions
 

Mowiwol Preparation

Author: Amit Sharma, updated date: , view: 61, Q&A: 0
Tags: Cell Culture and Microscopy

Materials and Reagents

  1. Mowiol (Mowiol 4.88, Calbiochem catalog number 475904)

  2. glycerol

  3. NaN3 (Sodium Azide)

Procedure

  1. Add 6 g glycerol in a 50 ml plastic centrifuge tube and mix will using a small stir bar on megnatic sterring.

  2. Add 2.4 g Mowiol (Calbiochem); stir to mix.

  3. While stirring, add 6 ml distilled water and leave 2 hours at room temperature

  4. Add 12 ml 0.2M Tris (pH 8.5).

  5. Add a small amount of NaN3 (Sodium Azide) such that the final concentration is .02% (optional).

  6. Incubate the tube in hot water (50 – 60ºC) for 10 minutes to dissolve the Mowiol. This can be repeated over several hours if necessary

  7. Centrifuge at 5000 g for 15 minutes to remove any undissolved solids. Store 1ml aliquots in eppendorf tubes at -20ºC.

  8. Warm tubes to room temperature for use. Opened tubes can be stored at 4ºC for approximately 1 month. Discard if any crystalline material is seen in the tube or on the slides.


Comment
Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.
You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.