Materials and Reagents
Mowiol (Mowiol 4.88, Calbiochem catalog number 475904)
glycerol
NaN3 (Sodium Azide)
Procedure
Add 6 g glycerol in a 50 ml plastic centrifuge tube and mix will using a small stir bar on megnatic sterring.
Add 2.4 g Mowiol (Calbiochem); stir to mix.
While stirring, add 6 ml distilled water and leave 2 hours at room temperature
Add 12 ml 0.2M Tris (pH 8.5).
Add a small amount of NaN3 (Sodium Azide) such that the final concentration is .02% (optional).
Incubate the tube in hot water (50 – 60ºC) for 10 minutes to dissolve the Mowiol. This can be repeated over several hours if necessary
Centrifuge at 5000 g for 15 minutes to remove any undissolved solids. Store 1ml aliquots in eppendorf tubes at -20ºC.
Warm tubes to room temperature for use. Opened tubes can be stored at 4ºC for approximately 1 month. Discard if any crystalline material is seen in the tube or on the slides.