HCA2 or IMR-90 (early passage cells)
Lentivirus (containing shRNA – GFP Ctrl and target gene)
Growth medium: DMEM, 10% FBS, 1% antibiotic – warm to 37°C
Freezing medium: 90% growth medium + 10% DMSO
Trypsin – warm to 37°C
1X PBS (without calcium) – warm to 37°C
15 ml tubes, 10% bleach