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Flow Cytometry (with Methanol)
Author: Alex Kuo, updated date: , view: 4, Q&A: 0
Tags: CyTOF and Flow Cytometry

Procedure

FLOW CYTOMETRY with TRITON


Wash cells with PBS


Trypsinize cells, collect in media, and spin down at 300g for 5 min


Resuspend in PBS and spin


Add 1 ul Zombie for 1 million cells


Incubate 15 mins in dark


Wash w/CyTOF buffer and spin


Add 4 mL 1.6% PFA in PBS and incubate for 15 mins


Add 1 mL 0.1% Triton in PBS and incubate for 20 mins


Wash twice at 600g for 5 mins


Add blocking and antibodies


Incubate overnight


Wash twice with CyTOF buffer



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